![]() ![]() Capillary Blotting of Glycosaminoglycans on Nitrocellulose Membranes after Agarose-gel Electrophoresis Separation- Nicola Volpi and Francesca Maccari MODIFICATIONS OF ELECTROBLOTTING 13. Dot-Immunobinding Assay (Dot-Iba) - Sumi Surendran, Annamma Mathai and Vishnampet Venkataraman Radhakrishnan 12. Localizing Proteins by Tissue Printing - Rafael Pont-Lezica 11. ![]() Non-electrophoretic bi-directional transfer of a single SDS-PAGE gel with multiple antigens to obtain twelve immunoblots - Biji T. Affinity immunoblotting for analysis of antibody clonotype distribution in a lupus patient developing anti-Ro 60 over time-Biji T. Tryptic peptide purification using polyvinylidene difluoride membrane for matrix assisted laser desorption ionization time of flight mass spectrometry -Biji T. Diffusion blotting for rapid production of multiple identical imprints from sodium dodecyl sulfate polyacrylamide gel electrophoresis on a solid support- Ingrid Olsen and Harald G. Simultaneous Immunoblotting Analysis with Activity Gel Electrophoresis and 2-D Gel Electrophoresis- Der-Yen Lee and Geen-Dong Chang 5. Introduction to protein blotting- Biji T. Western Blotting: Remembrance of Things Past-W. Hal Scofield, MD Table of Contents Preface- R. Protein Blotting and Detection: Methods and Protocols Editors Biji T. In this volume, expert researchers present numerous techniques based on the Western blot, providing detailed, readily reproducible methods, tips and alternatives directly and easily transferable to the laboratory setting.Ĭhapters offer a large number of variations on the theme of protein transfer to solid support followed by detection, presenting both adaptations of traditional techniques as well as completely original methods of protein blotting. Rapid Hybridization System-Multiprime, protocol booklet (1988) Amersham International plc.Over the past thirty years, the development of the Western blot has revolutionized the fields of biomedical research and medical diagnostics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. (1982) Molecular Cloning: A Laboratory Manual. (ed.) (1984) Methods in Molecular Biology, vol. J., eds.), IRL, Oxford and Washington DC, pp. (1985) Quantitative filter hybridization, in Nucleic Acid Hybridization: A Practical Approach (Hames, B. 9: Protocols in Human Molecular Genetics (Mathew, C. (1991) The detection of specific DNA sequences by enhanced chemiluminescence, in Methods in Molecular Biology, vol. (1984) Detection of mRNAs in sea urchin embryos by in situ hybridization using asymmetric RNA probes. (1984) Addendum: A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. (1983) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. (1990) Filter hybridization and radiolabelling of nucleic acids, in Advances in Gene Technology vol. (1985) Rapid transfer of DNA from agarose gels to nylon membranes. (1984) Hybridization of nucleic acids immobilized on solid supports. (1988) Vacuum blotting enhances nucleic acid transfer. (1984) Detection of specific sequences-the Southern Transfer, in Methods in Molecular Biology, vol. (1991) Diagnosis of genetic disorders with linked DNA markers, in Methods in Molecular Biology, vol. ![]() (1991) DNA fingerprinting analysis: Methodology and its applications, in Methods in Molecular Biology, vol. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. ![]()
0 Comments
Leave a Reply. |